Home > Explore Data & Reports > Proteome changes driven by phosphorous deficiency and recovery in the brown tide-forming alga Aureococcus anophagefferens

Citation:

Wurch, L.L., E.M. Bertrand, M.A. Saito, B.A.S. Van Mooy, and S.T. Dyhrman. 2011. Proteome changes driven by phosphorous deficiency and recovery in the brown tide-forming alga Aureococcus anophagefferens. PLoS ONE, 6(12):e28949. https://doi.org/10.1371/journal.pone.0028949

Data/Report Type:

Sponsored Research

Description

Shotgun mass spectrometry was used to detect proteins in the harmful alga, Aureococcus anophagefferens, and monitor their relative abundance across nutrient replete (control), phosphate-deficient (?P) and ?P refed with phosphate (P-refed) conditions. Spectral counting techniques identified differentially abundant proteins and demonstrated that under phosphate deficiency, A. anophagefferens increases proteins involved in both inorganic and organic phosphorus (P) scavenging, including a phosphate transporter, 5?-nucleotidase, and alkaline phosphatase. Additionally, an increase in abundance of a sulfolipid biosynthesis protein was detected in ?P and P-refed conditions. Analysis of the polar membrane lipids showed that cellular concentrations of the sulfolipid sulphoquinovosyldiacylglycerol (SQDG) were nearly two-fold greater in the ?P condition versus the control condition, while cellular phospholipids were approximately 8-fold less. Transcript and protein abundances were more tightly coupled for gene products involved in P metabolism compared to those involved in a range of other metabolic functions. Comparison of protein abundances between the ?P and P-refed conditions identified differences in the timing of protein degradation and turnover. This suggests that culture studies examining nutrient starvation responses will be valuable in interpreting protein abundance patterns for cellular nutritional status and history in metaproteomic datasets.

Note to readers with disabilities: Some scientific publications linked from this website may not conform to Section 508 accessibility standards due to the complexity of the information being presented. If you need assistance accessing this electronic content, please contact the lead/corresponding author, Primary Contact, or nccos.webcontent@noaa.gov.

Explore Similar Data/Reports
NCCOS-with-tag-to-side-bld

NCCOS delivers ecosystem science solutions for stewardship of the nation’s ocean and coastal resources, in direct support of NOS priorities, offices, and customers, and to sustain thriving coastal communities and economies.

National Centers for Coastal Ocean Science
1305 East West Highway, Rm 8110
Silver Spring, MD 20910
Phone: (240) 533-0300 / Fax: (301) 713-4353
Email: nccos.webcontent@noaa.gov

    Sign Up for Our Quarterly Newsletter