This report summarizes the remotely operated vehicle (ROV) surveys during four cruises from 2012 to 2015 which characterizes the mesophotic coral reef ecosystems at Pulley Ridge and Tortugas. This research is part of a grant funded by the NOAA National Centers for Coastal Ocean Science award NA11NOS4780045: “Connectivity of the Pulley Ridge - South Florida Coral Reef Ecosystem”. The University of Miami ship R/V Walton Smith was used along with the University of North Carolina at Wilmington (UNCW) Super Phantom ROV and the National Marine Sanctuary Foundation (NMSF) Mohawk ROV. The cruises were a collaboration of the University of Miami, HBOI-CIOERT, NOAA Fisheries, and the UNCW-CIOERT Undersea Vehicles Program. This project documents and characterizes the mesophotic benthic habitat, benthic macrobiota, and fish populations within and adjacent to Pulley Ridge Habitat Area of Particular Concern (PR HAPC) and at sites adjacent to, but outside, of the North and South Tortugas Ecological Reserves (TERs), and the Florida Keys National Marine Sanctuary (FKNMS). Ultimately these data may be used to better understand the long-term health and status of these important mesophotic ecosystems. These data will be of value to the regional Fishery Management Councils, NOAA OER, NOAA NCCOS, NOAA Fisheries, NOAA Mesophotic Reef Ecosystem Program, NOAA Deep-sea Coral Research and Technology Program (DSCRTP), NOAA Coral Reef Conservation Program (CRCP), and NOAA Marine Sanctuaries for management decisions on these habitats and managed key species. This report summarizes the ROV data that were collected within 68 1-km2 random Blocks that were surveyed over all 4 years at the Pulley Ridge (PR) mesophotic reef, and 23 Blocks in the region of the Tortugas (TOR) during our cruises in 2013 and 2014. Individual cruise reports (Reed et al. 2012a, 2012b, 2012c, 2014, 2015, 2016a, 2017) for each cruise provided detailed SEADESC (Southeastern United States Deep-Sea Corals) characterization of the benthic habitat, benthic macrobiota, and fish populations for each dive site