Publication Details
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HANDHELD NASBA-BASED SENSORS FOR RED TIDE DETECTION
Author(s): Paul, John; Robert Ulrich
NCCOS Center: CSCOR
Name of Publisher: PureMolecular LLC
Place of Publication: St. Petersburg, FL
Publication Type: Fact Sheet
Date of Publication: 2017
Reference Information: PureMolecular LLC product fact sheet
Extent of Work: 1 p.
Keywords: NASBA; red tide; sensors; K. brevis; tricorder; HAB
Abstract: This fact sheet describes a patented process for nucleic acid sequence based amplification (NASBA). NASBA produces results similar to PCR in terms of amplification of specific nucleic acid sequences via an enzymatic reaction. However, NASBA is an isothermal amplification protocol (i.e. does not require temperature cycling) that simplifies the hardware requirements; the process does not require a thermostable DNA polymerase or a thermal cycler. Secondly, NASBA works on RNA rather than DNA., inherently increasing sensitivity and enabling transcript (mRNA) abundance detection, an estimate of gene expression. NASBA technology has been incorporated into portable and hand held sensors to provide more timely and sensitive enumeration of harmful algal bloom species such as Karenia brevis, the Florida red tide alga.
Availability: Available from NCCOS Publications Explorer and from the publisher.
Related Attachment: Download file (.pdf)
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