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DATA/REPORT DETAILS

Histological Techniques for Marine Bivalve Molluscs: Update

Citation:
Kim, Y., K.A. Ashton-Alcox, and E.N. Powell
Data/Report Type:
NOAA Technical Memorandum

Description

This chapter describes the procedures for determining the reproductive stage of oysters, mytilid mussels, and dreissenid mussels collected for NOAA’s National Status and Trends Mussel Watch Project. Analyses are conducted on paraffin-embedded tissues sectioned at a 5-µm thickness and stained using a pentachrome staining procedure. Each slide is examined microscopically to determine the animal’s sex and stage of gonadal development. A semi-quantitative ranking is assigned.

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