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Population DNA Distribution, Cellular DNA Content, and the Diel DNA Cell Cycle of Cultured Karlodinium spp. (Dinophyceae)
Author(s): Parrow, Matthew W.; Laurie E. Barnes;JoAnn M. Burkholder
NCCOS Center: CSCOR
Name of Publisher: WHOI
Place of Publication: Woods Hole, MA
Publication Type: Abstract
Journal Title: Proceedings of 3rd Symposium on Harmful Algae in the U.S., Pacific Grove, CA.
Date of Publication: 2005
Keywords: Karlodinium veneficum; diel cycle; DNA; cell cycle; population distribution
Abstract: Seven strains of Karlodinium veneficum and one strain of Karlodinium cf. armiger were examined for DNA content by flow cytometric analysis of cells stained with the DNA fluorophore SYBR Green. Each strain exhibited distinct 1C, intermediate, and 2C DNA subpopulations indicative of respective eukaryotic cell cycle phases G1, S, and G2M. The estimated 1C DNA content of K. armiger (GC-3) was 32.9 ± 2.4 pg cell-1, a significantly higher amount than was found in K. veneficum. It was unknown whether chromosome number also varied with DNA content. Cells with two longitudinal flagella, a feature generally attributed to dinoflagellate planozygotes, were consistently observed in Karlodinium spp. cultures. Thus, some cells with 2C DNA may have formed by gamete fusion rather than DNA synthesis. If persistent sexuality occurred, the observed lack of 4C DNA cells could have implications for the meiotic process in Karlodinium spp. Alternatively, Karlodinium vegetative cells may develop two longitudinal flagella prior to asexual division, as was originally reported for this dinoflagellate more than 40 years ago [1,2]. This possibility requires further study.
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