Legacy Publications Explorer

You are here: Publications / Publication Details

Publication Details

Please note this explorer contains 2017 and prior publications and is no longer updated. Visit Data Reports Explorer for the latest NCCOS research data and reports.

Population DNA Distribution, Cellular DNA Content, and the Diel DNA Cell Cycle of Cultured Karlodinium spp. (Dinophyceae)

Author(s): Parrow, Matthew W.; Laurie E. Barnes;JoAnn M. Burkholder

NCCOS Center: CSCOR

Name of Publisher: WHOI

Place of Publication: Woods Hole, MA

Publication Type: Abstract

Journal Title: Proceedings of 3rd Symposium on Harmful Algae in the U.S., Pacific Grove, CA.

Date of Publication: 2005

Reference Information: p. 53

Keywords: Karlodinium veneficum; diel cycle; DNA; cell cycle; population distribution

Abstract: Seven strains of Karlodinium veneficum and one strain of Karlodinium cf. armiger were examined for DNA content by flow cytometric analysis of cells stained with the DNA fluorophore SYBR Green. Each strain exhibited distinct 1C, intermediate, and 2C DNA subpopulations indicative of respective eukaryotic cell cycle phases G1, S, and G2M. The estimated 1C DNA content of K. armiger (GC-3) was 32.9 ± 2.4 pg cell-1, a significantly higher amount than was found in K. veneficum. It was unknown whether chromosome number also varied with DNA content. Cells with two longitudinal flagella, a feature generally attributed to dinoflagellate planozygotes, were consistently observed in Karlodinium spp. cultures. Thus, some cells with 2C DNA may have formed by gamete fusion rather than DNA synthesis. If persistent sexuality occurred, the observed lack of 4C DNA cells could have implications for the meiotic process in Karlodinium spp. Alternatively, Karlodinium vegetative cells may develop two longitudinal flagella prior to asexual division, as was originally reported for this dinoflagellate more than 40 years ago [1,2]. This possibility requires further study.

Availability: Available from NCCOS Publications Explorer and from the authors.

Related Attachment: Download file (.pdf)


Note to readers with disabilities: Some scientific publications linked from this website may not conform to Section 508 accessibility standards due to the complexity of the information being presented. If you need assistance accessing this electronic content, please contact the lead/corresponding author, Primary Contact, or nccos.webcontent@noaa.gov.